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KMID : 0880220170550050366
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Journal of Microbiology
2017 Volume.55 No. 5 p.366 ~ p.372
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Minimal amino acids in the I/LWEQ domain required for anterior/posterior localization in Dictyostelium
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Kim Hye-Seon
Shin Dong-Yeop
Jeon Taeck-Joong
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Abstract
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Establishment of cell polarity is mediated by a series of signaling molecules that are asymmetrically activated or localized in the cell upon extracellular stimulation. To understand the mechanism that mediates anterior/posterior asymmetric localization of RapGAP3 during migration, we determined the minimally required amino acids in the I/LWEQ domain that cause posterior localization and found that the minimal region of the F-actin binding domain for posterior localization could, with some additional deletion at the C-terminal, localize to the anterior. Analysis of the localization and translocation kinetics to the cell cortex of the truncated proteins suggests that the required regions for anterior/posterior localization might have a preferential binding affinity to preexisting F-actins at the rear and lateral sides of the cell or newly formed F-actins at the front of the cell, leading to distinct differential sites of the cell.
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KEYWORD
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cell polarity, cell migration, Dictyostelium, I/LWEQ domain, RapGAP3
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